Fast DNA-based identification of the black truffle Tuber melanosporum with direct PCR and species-specific primers

Abstract

A quick and sensitive DNA-based assay for identifying the black truffle species Tuber melanosporum is presented. Both direct PCR and species-specific primers are used to amplify a phylogenetically informative region of T. melanosporum rDNA. This method was successfully used to screen fresh and frozen T. melanosporum fruitbodies, and could be modified for the molecular detection of other truffle and mushroom species. © 2009 Federation of European Microbiological Societies.