Microsatellite markers for Eucalyptus pilularis (Subgenus Eucalyptus); sourcing genetic markers outside the subgenus

Abstract

Microsatellite markers remain the most broadly used molecular marker in eucalypt genetics. A major advantage of microsatellite markers is that they often transfer readily between related taxa circumventing the need to develop new markers de novo in each species. Markers have been developed for a number of species of major economic importance, mainly from the Subgenus Symphyomyrtus, but these may also be available for use in species of lesser economic importance from other subgenera. Here we report on the sourcing of microsatellite markers for E. pilularis (Subgenus Eucalyptus (Formerly Monocalyptus)) from species outside the subgenus. Ninety-seven precent (60 out of 62) of loci that amplified in the source taxon (E. grandis) also amplified in the target taxon E. pilularis. By characterising them on a diversity panel (n=24) and a pedigree, a subset of 41 loci were distilled out that could be scored reliably and were polymorphic (Mean unbiased heterozygosity=0.81). Predictions of efficient microsatellite marker transfer among eucalypts based on low evolutionary divergence have largely been borne out and are congruent with accumulating evidence of low sequence divergence within Eucalyptus. Upon this favourable background for microsatellite marker transfer, this study indicates highly efficient transfer is possible by identifying loci with broad PCR optima and adoption of approaches that favour cross-species transfer.