Effects of daidzein on estrogen-receptor-positive and negative pancreatic cancer cells in vitro

Abstract

Aim: To study the effects of daidzein on human pancreatic cancer cells in vitro. Methods: Human estrogen-receptor (ER)-positive pancreatic cancer cells MiaPaCa-2 and ER-negative pancreatic cancer cells PANC-1 were treated by 0.1 μmol/L, 1 μmol/L, 10 μmol/L, 25 μmol/L, 50 μmol/L, 75 μmol/L and 100 μmol/L of daidzein, respectively. Its antiproliferative effect was studied by MTT assay. Results: Daidzein inhibited the growth of MiaPaCa-2 and PANC-1 cells at the concentrations from 0.1 μmol/L to 100 μmol/L. A dose- and time-dependent manner was found. The IC50 of daidzein on MiaPaCa-2 and PANC-1 cells was 45 μmol/L and 75 μmol/L, respectively. After MiaPaCa-2 cells were treated by daidzein for 3 d and at the concentrations more than IC50, the inhibitory manner was identical and the inhibition appeared a saturation phenomenon, but the inhibitory manner of daidzein on PANC-1 cells was different from that of MiaPaCa-2 cells. Conclusion: Daidzein has antiproliferative effects on human estrogen-receptor-positive and negative pancreatic cancer cells, but their mechanisms may be different. Copyright © 2004 by The WJG Press.