Hypoglycemic activity and pancreas protection of combination of Morinda citrifolia Linn. juice and Curcuma xanthorrhiza Roxb. juice on streptozotocin-induced diabetic rats

Abstract

Mengkudu fruit and temulawak rhizome which contains scopoletin and curcumin, respectively, were used traditionally as antidiabetic. Both compounds have strong antioxidant activity. This research aimed to determined the antihyperglycemic and pancreas protection of combination of mengkudu fruit juice (MFJ) and temulawak rhizome juice (TRJ) on streptozotocin (STZ) induced diabetic rats. Rats were divided into 7 groups, each group consist of 5 rats. All groups, except normal group were treated accordingly for 28 days. Blood samples were taken from the plexus retroorbitalis for examination of blood glucose levels on day-8th, 15th, 22nd, and 29th. On the day-29th, blood samples were examined for malondialdehyde (MDA) levels. At the end of the experiment, the rats were sacrificed for examination of pancreatic morphological conditions. The results showed that diabetic rats given MFJ-TRJ combination experienced a significant decrease in blood glucose levels, a significant decrease in MDA levels and improvement pancreas morphology when compared with the negative control group. The conclusion of this research showed the combination of MFJ-TRJ has hypoglycemic activity and can fix condition of pancreas morphology.