Enzymatic Synthesis of M 1 G‐Deoxyribose

Abstract

Adducts formed between electrophiles and nucleic acid bases are believed to play a key role in chemically induced mutations and cancer. M 1 G‐dR is an endogenous exocyclic DNA adduct formed by the reaction of the dicarbonyl compound malondialdehyde with a dG residue in DNA. It is an intermediate in the synthesis of a class of modified oligodeoxyribonucleotides that are used to study the mutagenicity and repair of M 1 G. This unit presents methods for synthesizing M 1 G‐dR by enzymatic coupling.