Isolation of an atl type-IV collagen cDNA clone using a synthetic oligodeoxynucleotide

Abstract

We have isolated a cDNA clone (pCIV-1-225) for the α1 subunit of basement membrane (type IV) collagen from a cDNA library made from Engelbreth-Holm-Swarm mouse tumor RNA. The cDNA library was screened with synthetic oligodeoxynucleotides derived from published amino acid (aa) sequences (Schuppan et al., 1982). Nucleotide sequence data established the identity of our cDNA clone to encode an α1 type IV collagen. This clone contains 270 aa of the helical region and has three interruptions in the Gly-X-Y repeat unit. © 1986.