Separation and detection of polar cuticular components from Oriental tobacco leaf by integration of normal-phase liquid chromatography fractionation with reversed-phase liquid chromatography-mass spectrometry

Abstract

Integration of normal-phase LC (NPLC) fractionation with RPLC-ESI/MS was established to detect the polar fractions of cuticular components from Oriental tobacco leaf. NPLC was selected for the fractionation of polar components of cuticular leaf extract, after being concentrated with rotary evaporator, each of the enriched fractions was further analyzed by RPLC-ESI/MS. In total, 83 compounds were finally detected including 45 cembranoids, 15 labdanoids, 20 sucrose esters, and 3 glucose esters (or fructose esters). Three cembranoids and seven labdanoids possibly are new diterpenoids. Glucose esters (or fructose esters) are also reported in Nicotiana tobacco for the first time. © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.