Structural properties, cytotoxicity, and anti-inflammatory activity of silver(I) Complexes with tris(p-tolyl)Phosphine and 5-Chloro-2- mercaptobenzothiazole

Abstract

The synthesis and characterization of the silver(I) chloride complex of formula { [ AgCI(CMBZT) (TPTP)2 ]̇(MeOH) } (1) (CMBZT = 5-chloro-2-mercaptobenzothiazole, TPTP = tris(p-tolyl)phosphine) is described. Also the structure of the hydrate derivative { [ AgCI (TPTP)3 ]̇(0.5̇ H2 O) } (2) of the corresponding known anhydrous silver complex (Zartilas et al., 2009), and the polymorph 3 of the known [ AgI (TPTP)3 ] complex (Zartilas et al., 2009) were determined and compared with the known ones. In addition, the structure of the known one silver(I) cluster { [ AgI(TPTP) ] 4 } (4) (Meijboom et al., 2009) was re-determined at 120(2) K and possible Ag-Ag interactions were analyzed. The compounds 1-4 were characterized by X-ray crystallography at r.t (1) and 120 K (2-4). All these complexes and { [ (Et3 NH) + ] 2 [ Ag6 ( μ3 -Hmna)4 ( μ3-mna)2 ]2- (DMSO)2 (H 2 O) } (5) (Hmna = 2-mercaptonicotinic acid) were evaluated for cytotoxic and anti-inflammatory activity. The in vitro testing of cytotoxic activity of 1-5 against leiomyosarcoma cancer cells (LMS), were evaluated with Trypan Blue and Thiazolyl Blue Tetrazolium Bromide or 3-(4.5-dimethylthiazol-2- yl)-2.5-diphenyltetrazolium bromide (MTT) assays. The flow cytometry assay for complex 1 and showed that at 15μM of 1, 62.38 of LMS cells undergo apoptosis, while 7 of LMS cells undergo cell necrosis. The antitumor activity of 3 is comparable with that of its reported polymorph (Zartilas et al., 2009). The anti-inflammatory, activity of complexes 1-3 and 5 was also studied. The activity towards cell viability was 2>3>5>1>4, while the order of the inhibitory activity in cell growth proliferation follows the order, 2>3>1>4>5. The anti-inflammatory activity on the other hand is 1>2>5>⋯>3. © 2010 L. Kyros et al.