Synthesis, Characterization, Anticancer, DNA Binding and Antioxidant Studies of Benzylamine Supported Pd (II) Complex

Abstract

dryness. The TEM images reports from the SAED pattern was taken at different magnification suggesting the presence of different elements which are semi crystalline and homogeneous in shape and size (ESI figures S4). The proper alignment is not seen in the rings which indicate that the complex is not completely crystalline but a clear formation of rings suggesting the semi crystalline nature of complexes. Anticancer activity The complexes were tested in vitro against MCF-7 and MDA-MB-231 human breast tumour cell lines by colorimetric micro culture 2-(3-diethylamino-6-diethylazaniumylidene-xanthen-9-yl)-5-sulfo-1H NMR, the 2H of -NH2 and PhCH2-appeared at δ 3.93 to 2.086 and 5.17 to 3.85 with singlet for the synthesized complex. In complex, the 2H of C3 and C7 showed a doublet at δ 7.29-7.28 with J=6.5 MHz where a triplet of 2H of C4 and C6 was found at δ 7.45-7.47 having J= 7.3 MHz. A multiplet of H of C5 was found at δ 7.49-7.51. In 13C NMR, the benzyl carbon C1 at δ 47.84, C2 at 138.34, C3 and C7 found at 127.57-127.14, C4 and C6 found between 128.73-128.36 and C5 found at 128.1 (ESI Figures S1 and S2) [21,22]. The +ve ESI mass spectra of Pd complex have found [M+1] for complex confirming its molecular mass. To investigate a solid state structure retained in solution, the UV/ Vis spectral behaviour was investigated in DMSO, DMSO+water as well as in DMSO+phosphate buffer for the complex (Figures 1-3). The overall patterns of spectra for complexes solution were found similar with the different mediums to ensure their sustainability in liquid state. The UV/Vis absorption from 265 to 270 nm and 1H NMR coupling constant between 5 and 9 MHz, have confirmed their trans geometry [18,23,24].