Effect of H 2S on the circadian rhythm of mouse hepatocytes

Abstract

Background: Dysregulation of circadian rhythms can contribute to diseases of lipid metabolism. NAD-dependent deacetylase sirtuin-1(SIRT1) is an important hub which links lipid metabolism with circadian clock by its deacetylation activity depends on intracellular NAD +/NADH content ratio. Hydrogen sulfide (H 2S) is an endogenous reductant which can affect the intracellular redox state. Therefore, we hypothesized that exogenous H 2S can affect the expression of circadian clock genes mediated by sirt1 thereby affecting body's lipid metabolism. And also because the liver is a typical peripheral circadian clock oscillator that is intimately linked to lipid metabolism. Thus the effect of H 2S were observed on 24-hour dynamic expression of 4 central circadian clock genes and sirt1gene in primary cultured hepatocytes. Results: We established a hepatocyte model that showed a circadian rhythm by serum shock method. And detected that the expression level and the peak of circadian clock genes decreased gradually and H 2S could maintain the expression and amplitude of circadian clock genes such as Clock, Per2, Bmal1 and Rev-erbwithin a certain period time. Accordingly the expression level of sirt1 in H 2S group was significantly higher than that in the control group. Conclusion: Exogenous reductant H 2S maintain the circadian rhythm of clock gene in isolated liver cells. We speculated that H 2S has changed NAD +/NADH content ratio in hepatocytes and enhanced the activity of SIRT1 protein directly or indirectly, so as to maintain the rhythm of expression of circadian clock genes, they play a role in the prevention and treatment of lipid metabolism-related disease caused by the biological clock disorders. © 2012 Shang et al; licensee BioMed Central Ltd.