Background: There are several groups of viruses including Insect Specific Viruses (ISV) such as the taxon Negevirus, a group of viruses phylogenetically related to plant viruses. Negeviruses replicate in mosquito cells, but not in vertebrate cells. Methods: Pools of hematophagous arthropods were inoculated in Vero and C6/36 cells. The cells were observed to detect possible cytopathic effect. Then, indirect immunofluorescence, RT-PCR, and nucleotide sequencing were performed. Results: Seven samples which presented negative results for flaviviruses, alphaviruses and bunyaviruses, but showed cytopathic effect in C6/36 cells were sequenced. We identified the occurrence of a variety of ISVs, most of them belonging to the taxon Negevirus: The Brejeira, Negev, Cordoba and Wallerfield viruses, including a new virus for science, tentatively named Feitosa virus. Conclusions: We detected negeviruses in the Amazon region, including two viruses that were isolated for the first time in Brazil: Cordoba virus and the Negev virus and, a new virus for science: the Feitosa virus.
CITATION STYLE
da Silva Ribeiro, A. C., Martins, L. C., da Silva, S. P., de Almeida Medeiros, D. B., Miranda, K. K. P., Nunes Neto, J. P., … Rodrigues, S. G. (2022). Negeviruses isolated from mosquitoes in the Brazilian Amazon. Virology Journal, 19(1). https://doi.org/10.1186/s12985-022-01743-z
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