Spirulina -PCL Nanofiber Wound Dressing to Improve Cutaneous Wound Healing by Enhancing Antioxidative Mechanism

Abstract

Skin regeneration is a complex process involving massive proliferation and alignment of cells, where there are obstacles to completion of regeneration, the main one being excessive generation of reactive oxygen species (ROS) from inflammation or infection. Spirulina, blue-green algae that has antioxidant and anti-inflammatory activities, has been used to relieve such ROS stress. In this study, Spirulina extract loaded PCL (Spirulina-PCL) nanofiber was evaluated as a cutaneous wound dressing in view of antioxidative mechanism. In addition to increasing fibroblast viability, the Spirulina extract and its dressing modulated intra- and extracellular ROS by enhancing antioxidant mechanism of fibroblast under oxidative stress. Finally, in vivo assays confirmed that Spirulina-PCL helps regenerate wounds and enhance regeneration. Taken together, the results of this study indicate that Spirulina and nanofiber have the potential for application to cutaneous wound to facilitate skin regeneration.