Chronic pancreatitis in mice by treatment with choline-deficient ethionine-supplemented diet

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Abstract

Although chronic pancreatitis is a risk factor for pancreatic ductal adenocarcinoma (PDA), the relationship between chronic pancreatitis and PDA remains obscure. A critical obstacle to understanding the role of chronic pancreatitis is the lack of animal models. To develop one such model, mice were fed long-term with a choline deficient ethionine- supplemented (CDE) diet. Histological evaluation revealed that chronic pancreatitis, characterized by acinar atrophy, fibrosis and well-developed tubular complexes (TCs), was observed after 24 weeks of CDE diet treatment. Furthermore, expression of epidermal growth factor receptor (EGFR) and its ligands; serine protease inhibitor Kazal type 3 (Spink3) and transforming growth factor a (TGF a) and activation of K-Ras (GTP-Ras formation), which are frequently observed in human PDA, were indeed observed in parallel with TCs formation. Neoplastic lesions were not found after 54 weeks of treatment, suggesting that a continuation of CDE diet or another insult is required for the development of PDA.

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Ida, S., Ohmuraya, M., Hirota, M., Ozaki, N., Hiramatsu, S., Uehara, H., … Yamamura, K. ichi. (2010). Chronic pancreatitis in mice by treatment with choline-deficient ethionine-supplemented diet. Experimental Animals, 59(4), 421–429. https://doi.org/10.1538/expanim.59.421

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