Kinetics of NH4+ influx in spruce

Abstract

Influxes of 13NH4+ across the root plasmalemma were measured in intact seedlings of Picea glauca (Moench) Voss. Two kinetically distinct uptake systems for NH4+ were identified. In N-deprived plants, a Michaelis-Menten-type high-affinity transport system (HATS) operated in a 2.5 to 350 μM range of external NH4+ concentration ([NH4+]o). The Vmax of this HATS was 1.9 to 2.4 μmol g-1 h-1, and the Km, was 20 to 40 μM. At [NH4+Jo from 500 μM to 50 mM, a linear low-affinity system (LATS) was apparent. Both HATS and LATS were constitutive. A time-dependence study of NH4+ influx in previously N-deprived seedlings revealed a small transient increase of NH4+ influx after 24 h of exposure to 100 μM [NH4+]o. This was followed by a decline of influx to a steady-state value after 4 d. In seedlings exposed to 100 μM external NO3- concentration for 3 d, the Vmax for NH4+ uptake by HATS was increased approximately 30% compared to that found in N-deprived seedlings, whereas LATS was down-regulated. The present study defines the much higher uptake capacity for NH4+ than for NO3- in seedlings of this species.