Spiral scanning fiber-optic two-photon endomicroscopy with a double-cladding antiresonant fiber

Abstract

Two-photon endomicroscopy is an optical biopsy tool that satisfies clinical requirements for real-time subcellular-resolution imaging to assist pathological biopsy in diagnosis. Herein, we present a two-photon endomicroscopy system based on a piezoelectric ceramic tube scanner. A dual-channel amplitude-modulated sine wave drives the fiber to realize spiral scanning, a double-cladding antiresonant fiber is used for 920-nm femtosecond light-pulse low dispersion transmission, and fluorescence collection occurs with no fiber-tip engineering. The field of view is ∼200 µm, the resolution is 3.1 µm, and the frame rate is 0.7 fps. Pollen grain, GFP-labeled mouse brain section, and human stomach tissue imaging verify the capability of the two-photon endomicroscopy system.