Identification of potato cyst nematode in Indonesia by polymerase chain reaction

Abstract

Polymerase chain reaction (PCR) with specific primers was used to amplify the ITS-rDNA regions of potato cyst nematodes (PCN) in soil samples from Indonesia. Amplified DNA fragments showed that a single species, Globodera rostochiensis, with fragment size 238 bp occurred in East Java, and West Java, while mixed populations of G. rostochiensis and G. pallida with fragment size 391 bp were only found in Central Java. © 2012 Australasian Plant Pathology Society Inc.