ACE, α-Glucosidase and Cancer Cell Growth Inhibitory Activities of Extracts and Fractions from Marine Microalgae, Nannochloropsis oculata

Abstract

Exts. of the marine microalgae Nannochloropsis oculata were obtained using 80% methanol (MeOH) and water. The 80% MeOH ext. was further fractionated with n-hexane, chloroform, Et acetate (EtOAc), n-butanol (n-BuOH), and water to isolate the active fraction. Seven samples were prepd. and their angiotensin converting enzyme (ACE), α-glucosidase, and cancer cell growth inhibitory activities in vitro were detd. The most profound ACE inhibitory activity was obsd. in the chloroform fraction, while the others had moderate effects. By contrast, greater α-glucosidase inhibitory activity was found in the EtOAc fraction, n-hexane fraction, and water ext. of N. oculata. The antiproliferative effects of the exts. and fractions against HL-60, U937, CT-26, and SK-Hep1 cancer cells were also detd. The n-BuOH fraction had the strongest antiproliferative effects on CT-26 cells in a time-dependent manner (P<0.05). These results suggest that the exts. and fractions from N. oculata could be used as a potential functional food or as pharmaceutical ingredients. [on SciFinder(R)]