Trichoderma sp., a potential producer for L-asparaginase isolated from Sonneratia alba inAeng Sareh Beach, Madura

Abstract

Mangrove ecosystem is a reservoir for natural product such as enzyme. One of the enzymes which able to be isolated from mangrove is L-asparaginase. The aim of this research was to obtain endophytic fungi which capable of producing L-asparaginase from mangrove. Sonneratia alba was obtained from Aeng Sareh Beach, Madura. Endophytic fungi were isolated from leaf, stem, and root of Sonneratia alba. The fungi were screened its capability to produce L-asparaginase using selective media namely modified Czapex Dox agar. The best producer of L-asparaginase was further identified with microscopic and macroscopic identification method. The result showed that seven strain produced L-asparaginase. Based on the analysis, Trichoderma sp. was the best producer for L-asparaginase.