Synergistic effects of olive oil pomace extract and mineral-medicinal waters in microparticle production for pathogen inhibition

Abstract

In recent years, the development of therapeutic agents or bioactive compounds from natural products has increased due to concerns about antimicrobial-resistant bacteria. This study investigates the antimicrobial properties of olive pomace extract (OP-extract) obtained through hydrodistillation and explores its incorporation into microparticles by spray-drying technology to enhance the stability of the OP-extract. Additionally, four mineral-medicinal waters (MMW) from province of Ourense (O Baniño de Arcos , Fonte de O Tinteiro , As Burgas and Parque de O Carballiño) and Milli-Q water (as a control) were studied as ingredients to explore the use of OP-extract microparticles as a natural dermatologic product. HS GC/MS analysis of OP-extracts revealed a volatile profile with over 20 compounds, mainly aldehydes. The antimicrobial activity of the OP-extract was tested against three pathogens, showing significant inhibition at a concentration of 20 %. Several carriers were evaluated to produce the OP-extract microparticles (10 %, w/w), identifying mannitol (1 %, w/w) as a suitable carrier. The incorporation of microparticles into MMW resulted in a consistent production yield of 30 %. For the three tested pathogens, inhibition was enhanced when microparticles were produced with Baniño MMW for two of them, with values around 81 % for C. albicans , 70 % for S. aureus , while for S. epidermis the value obtained was 65 %.