MiR-29a-3p represses proliferation and metastasis of gastric cancer cells via attenuating HAS3 levels

Abstract

MicroRNA-29a (miR-29a) has recently been in the spotlight as a tumor suppressor whose encoding gene is frequently suppressed in cancers. The aim of the present study was to investigate the biological functions and underlying molecular mechanism by which miR-29a-3p suppresses gastric cancer peritoneum metastasis. Cell proliferation, colony-forming, wound healing and Transwell migration assays were performed in the present study. MiR-29a-3p expression was markedly decreased in gastric cancer cell lines with stronger metastatic potential. Silencing miR-29a-3p expression promoted gastric cancer cell proliferation, colony-forming, migration and invasion. By contrast, overexpression of miR-29a-3p inhibited these biological phenotypes. In addition, it was revealed that miR-29a-3p functioned through downregulating hyaluronan synthase 3 expression. Collectively, dysregulated miR-29a-3p expression in gastric cancer cells was associated with malignant properties primarily relevant to migration and metastasis. The results suggest that miR-29a-3p may be a potential therapeutic target for gastric cancer.