The chromosome conformation capture (3C) technique is fundamental to many population-based methods investigating chromatin dynamics and organization in eukaryotes. Here, we provide a modified quantitative 3C (3C-qPCR) protocol for improved quantitative analyses of intra-chromosomal contacts. We also describe an algorithm for data normalization which allows more accurate comparisons between contact profiles.
CITATION STYLE
Ea, V., Court, F., & Forné, T. (2017). Quantitative analysis of intra-chromosomal contacts: The 3C-qPCR method. In Methods in Molecular Biology (Vol. 1589, pp. 75–88). Humana Press Inc. https://doi.org/10.1007/7651_2015_269
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