Effect of nutrient media and phytohormones on in vitro culture initiation of juno tratt. Species

Abstract

A long period of dormancy in seeds delay plant breeding. Using embryo culture technique, the breeding cycle can be shortened in plants. The aim of this experiment was breaking the seeds dormancy of Juno Tratt. species due to their structure peculiarities. The method of seed germination in vitro was appliedin order to accelerate plants reproduction and to obtain young and surface sterile plant material. Therefore, the calculation of optimum nutrient media for plant regeneration was conducted to provide maximum growth and development of explants and quality of developed plantlets. This work emphasizes on optimization of nutrient media. The significant effect of phytohormones (6-benzylaminopurine (BAP), indolyl-3-acetic acid (IAA), kinetin) at different concentrations and combinations during organ culture has been shown.