Thin layer chromatography of adenosine triphosphate in a pharmaceutical preparation

Abstract

The flexibility of adenosine triphosphate (ATP) with gradually decomposing structures has led to a study of the thin layer chromatography in the microgram region per 0.01 ml and the development of a method of separation for multipreparations containing amino acids, α-ketogulutaric acid and sugar. Randerath's method was examined. An empirical function of the area of the spot varies in position and shape as the amount of ATP and the thickness of the layer of DEAE-cellulose changes, and it was found that the spot of 15 μg/0.01 ml in the case of 0.5mm layer and the spot of 20 μg/0.01ml in 1 mm layer was the best. The method was applied successfully to a separation of the preparation of ATP containing ADP and AMP with widely varying distributions. The results indicate that this method can be applied with confidence to the separation of ATP, amino acids, α-ketogulutaric acids and sugar. © 1965, The Japanese Society of Physical Fitness and Sports Medicine. All rights reserved.