Recovery status of bacteriophages of different livestock farms of Veterinary College, Adhartal, Jabalpur, India

Abstract

Study was conducted to know the presence of bacteriophage in sewage material which can play a very important role during therapy against the some antibiotic resistance organisms. During study waste water samples were collected from different depths of the wastewater collection tanks located in livestock farms of different species (Cattle, pig, goat and poultry). These samples were subjected primarily to rapid detection by streak plate method for the detection of lytic activity followed by primary isolation of phage against two most common bacteria of environment, namely, B. subtilis and E. coli by Double agar layer (DAL) method. Recovery of phages was maximum from pig feces (67%) followed by dairy cattle farm waste (63%), buffalo farm waste (50%), goat farm waste (13%).