Evidence for bi-exponential transverse relaxation of lactate in excised rat muscle

Abstract

To elucidate the low proton nuclear magnetic resonance (NMR) visibility of muscle lactate previously demonstrated in excised rat muscle, lactate transverse relaxation was investigated in the same model using double quantum editing sequences with effective echo times ranging from 55 to 475 msec. On this time scale, muscle lactate clearly exhibits a bi-exponential transverse relaxation with a short T2 of 33 ± 5 msec (mean ± SE, n = 3) and a long T2 of 230 ± 10 msec. The relative populations (84 ± 4% vs. 16 ± 4%, respectively) of these two lactate pools are compatible with compartmentation between intra- and extracellular muscle lactate.