Interaction between Monobactams and Streptomyces R61 dd‐Carboxypeptidase

Abstract

The monobactams are a novel family of monocyclic β‐lactam antibiotics characterized by the 2‐oxoazetidine‐1‐sulfonic acid moiety. A series of monobactams bind covalently to the streptomyces R61 DD‐carboxypeptidase in a manner similar to that for bicyclic β‐lactams, especially cephalosporins. The similarity of interaction was established by the following criteria: inhibition of binding by diisopropylfluorophosphate and α‐dicarbonyls; stoichiometry of binding; similarity of partial proteolysis products of radiolabelled enzyme: rates of release of bound β‐lactams; nature of hydrolysis and hydroxylaminolysis products. Copyright © 1982, Wiley Blackwell. All rights reserved