Electrochemical immunosensor for monocyte chemoattractant protein-1 detection based on Pt nanoparticles functionalized single-walled carbon nanohorns

Abstract

A novel label-free electrochemical sensing strategy for detecting monocyte chemoattractant protein-1 (MCP-1) was developed by using single-walled carbon nanohorns (SWCNHs) functionalized with Pt nanoparticles (PtNPs-SWCNHs). After modification with the PtNPs-SWCNHs, the electrode could serve as an effective platform for antibody immobilization and electron transfer owing to the large surface area and excellent conductivity of PtNPs-SWCNHs. Moreover, the PtNPs-SWCNHs composite could provide an obvious electrochemical signal through amperometric i-t curve method, due to its high catalytic activity for the reduction of hydrogen peroxide (H2O2). Under optimal conditions, the amperometric i-t curve signals had a strong linear relation with the logarithm of the MCP-1 concentration ranging from 0.06 pg mL-1 to 450 pg mL-1, and the detection limit was 0.02 pg mL-1. Furthermore, the proposed immunosensor could detect MCP-1 in serum samples with desirable results. Overall, this immunosensor may provide a new alternative strategy for rapid detection of MCP-1.