Production of Polyhydroxyalkanoates by Bacillus megaterium: Prospecting on Rice Hull and Residual Glycerol Potential

Abstract

The production of polyhydroxyalkanoates (PHAs) by Bacillus megaterium using industrial residues, crude glycerol from biodiesel synthesis and rice hull hydrolysate (RHH), as low-cost carbon sources was investigated. The experiments were conducted by shaking flasks at 30 °C and 180 rpm up to 72 h. The extraction of PHA was carried out using sodium hypochlorite to make its recovery more environmentally friendly by avoiding organic solvents (chloroform). The yields of PHA varied depending on the extraction method. A total of 33.3% (w·w−1) (mixing chloroform: sodium hypochlorite) and 52.5% (w·w−1) (sodium hypochlorite only) were obtained using glycerol and glucose as a carbon source, respectively. Preliminary experiments using RHH as a carbon source Indicated a yield of PHA of 11% (w·w−1) (chloroform). The PHA produced had thermal properties, such as transition temperature, similar to the commercial polyhydroxybutyrate (PHB).