Deletion of PKBα/Akt1 affects thymic development

Abstract

Background. The thymus constitutes the primary lymphoid organ for the majority of T cells. The phosphatidyl-inositol 3 kinase (PI3K) signaling pathway is involved in lymphold development. Defects in single components of this pathway prevent thymocytes from progressing beyond early T cell developmental stages. Protein kinase 8 (PKB) is the main effector of the P13K pathway. Methodology/Principal Findings. To determine whether PKB mediates PI3K signaling in the thymus, we characterized PKB knockout thymi. Our results reveal a significant thymic hypocellularity in PKBα -/- neonates and an accumulation of early thymocyte subsets in PKBα-/- adult mice. Using thymic grafting and fetal liver cell transfer experiments, the latter finding was specifically attributed to the lack of PKBa within the lymphoid component of the thymus. Microarray analyses show that the absence of PKBα in early thymocyte subsets modifies the expression of genes known to be involved in pre-TCR signaling, in T cell activation, and in the transduction of interferon-mediated signals. Conclusions/Significance. This report highlights the specific requirements of PKBa for thymic development and opens up new prospects as to the mechanism downstream of PKBα in early thymocytes. © 2007 Fayard et al.