Deciphering protein-protein interactions. Part I. Experimental techniques and databases

Abstract

Proteins interact with each other in a highly speci.c manner, and protein interactions play a key role in many cellular processes; in particular, the distortion of protein interfaces may lead to the development of many diseases. To understand the mechanisms of protein recognition at the molecular level and to unravel the global picture of protein interactions in the cell, different experimental techniques have been developed. Some methods characterize individual protein interactions while others are advanced for screening interactions on a genomewide scale. In this review we describe different experimental techniques of protein interaction identication together with various databases which attempt to classify the large array of experimental data. We discuss the main promises and pitfalls of different methods and present several approaches to verify and validate the diverse experimental data produced by highthroughput techniques. © 2007 Shoemaker and Panchenko.