Rapid differentiation of cryptic species within Cryptococcus gattii by a duplex PCR assay

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Abstract

Cryptococcus gattii consists of four cryptic species, VGI, VGII, VGIII, and VGIV. Herein, a duplex PCR assay using two primer pairs targeting the vacuolar membrane gene and the intergenic spacer region was developed. It successfully distinguished the cryptic species according to the distinct size of the amplicons. Copyright © 2013, American Society for Microbiology. All Rights Reserved.

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Feng, X., Fu, X., Ling, B., Wang, L., Liao, W., Pan, W., & Yao, Z. (2013). Rapid differentiation of cryptic species within Cryptococcus gattii by a duplex PCR assay. Journal of Clinical Microbiology, 51(9), 3110–3112. https://doi.org/10.1128/JCM.01455-13

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