Effect of enterocins CCM4231 and V24 on the cells of environmental isolates acinetobacter spp

Abstract

The inhibitory effect of bacteriocins (enterocins) produced by different enterococcal isolates against Acinetobacter spp. strains AL1 and AL115 and/or on the resting cells of AL1 strain was tested under in vitro conditions. These poly-resistant strains (resistant to 6 from 12 antibiotics) were isolated from the cattle dung water and feces from chamois. Their growth was inhibited by treatment of crude extracts of enterocins CCM4231, V24, EC24, EK13 with activity ranged from 100 to 1600 Arbitrary units per ml. When log phase cells of Acinetobacter spp. AL1 strain were treated by crude extracts (CE) CCM4231 and V24 at temperatures 4°C and 33°C, the decrease of surviving cells in comparison to the control, one order of magnitude was found. In contrary, when exponential phase cells were harvested and treated by CE CCM4231 and V24, at 4°C no reduction was noted. While at 33°C the decrease of cells 2 orders and one order of magnitude was measured. Here, the effect of some enterocins against Gram-negative bacteria was confirmed as well as the way for further experiments to apply enterocins for the control and/or for maintaining the microbial balance in waste ecosystem.