Characterization of Rhizobium Isolated From Root Nodule of Pea (Pisum Sativum L)

Abstract

Studies were conducted on root nodules, collected from young and healthy seedling of pea, at research farm of soil science, Naini Agricultural Institute, Allahabad (SHUATS), U.P, India. The isolates were further subjected to morphological, biochemical and physiological (stress tolerance) characterization; three Rhizobium strains were isolated from the root nodule of field pea and characterized by standard biochemical tests. Isolated Rhizobia was fast grower, all strains were gram-negative rod and creamy color colony. Rhizobium strains showed difference in chemical test and urea hydrolysis, and the strains utilized glucose and mannitol as fermentation sugar, growth of Rhizobium strains tolerated to stress as temperature were at 20°C and 28°C, also observed well growth of sinorhizobium meliloti and rhizobium pusense strains at 2 % NaCl, while leguminosarum strain was grown at 3 % NaCl, and all strains were grown at pH 6 and pH 7. Optimum conditions help to enhance plant and super strain for fixed nitrogen in nodules of field pea crop