Neutralising antibody and challenge response to live and inactivated avian adenovirus-1 in broilers

Abstract

Immune responses to live and inactivated avian adenovirus-1 were evaluated in broilers by neutralising antibody response and challenge reaction. The neutralising antibody titre was 1 : 256, 1 : 64 and 1 : 32 in live virus, inactivated virus and uninoculated control birds respectively at 3 weeks post inoculation when they were challenged. At one week post challenge the antibody titres were 1 : 941, 1 : 247 and 1 : 375 in live virus, inactivated virus and control birds, respectively. There was a booster effect of challenge in the live virus inoculated group up to 3 weeks post challenge. The post challenge histopathological evaluation of liver, kidneys, lungs and bursa revealed less severe changes in the live virus inoculated group as compared to other groups.