The structure of the carbohydrate backbone of core-lipid A region of the lipopolysaccharides from Proteus mirabilis wild-type strain S1959 (serotype 03) and its Ra mutant R110/1959

Abstract

The following structure of core-lipid A region of the lipopolysaccharide (LPS) from Proteus mirabilis strain 1959 (serotype O3) and its rough mutant R110/1959 (Proteus type II core) was determined using NMR and chemical analysis of the core oligosaccharide, obtained by mild acid hydrolysis of LPS, and of the products of alkaline deacylation of the LPS. All sugars are in pyranose form, α-Hep is the residue L-glycero-α-D-manno-Hep, α-DD-Hep is the residue D-glycero-α-D-manno-Hep. The differences with the previously reported structures are discussed.