Restriction fragment length polymorphisms of rDNA and variation of essential oil composition in Atractylodes plants

Abstract

total DNA was extracted from the leaves of Atractylodes lancea De Candolle, A. ovata De Candolle and A. japonica Koidzumi ex Kitamura of various origins and hybridized with digoxigenin- labeled rice ribosomal DNA after digestion with eight different restriction endonucleases. The resulting restriction fragment length polymorphism (RFLP) profiles allowed us to distinguish the three Atractylodes species when DNA was digested with Sac1. Although atractylon was detected in the rhizomes of some of the cultivated strains of A. lancea, their RFLP profiles clearly indicate that these plants are not hybrids of A. ovata or A. japonica. RFLP analysis also revealed the presence of intraspecific variation in DNA sequence of rRNA locus among A. lancea as well as A. japonica.