Isolation and characterization of microsatellite loci in the grey mouse lemur (Microcebus murinus) and their amplification in the family Cheirogaleidae

Abstract

Within this study seven polymorphic microsatellite primers were developed for the grey mouse lemur (Microcebus murinus) from two partial genomic libraries. One protocol was based on an initial polymerase chain reaction (PCR) with random amplified polymorphic DNA (RAPD) primers and resulted in a 5.5% enrichment, whereas the second protocol included an enrichment with three rounds of capture which yielded a 38.0% enrichment. The genotypes of 12 captive and 162 free-living animals were determined with the newly developed primers and all seven confirmed Mendelian inheritance. All primers amplified in several species of the family Cheirogaleidae, demonstrating their general suitability for microsatellite analyses within this taxonomic group.