Optimized protocol for high-efficiency micropropagation of banana varieties G9 and Malbhog

Abstract

This research focuses on developing and improving micropropagation techniques for banana shoot tip culture, specifically aimed at enhancing cultivar production. Micropropagation allows for the rapid propagation of plants by cultivating small shoot sections in a controlled laboratory environment. The study investigates various factors, including media compositions, growth regulators, sterilization techniques, and environmental conditions, to optimize the success rate and efficiency of banana shoot tip culture. This work focused on two widely grown banana varieties in Bihar, India—G9 and Malbhog. Researchers adapted the standard Murashige and Skoog basal medium by adding carefully measured concentrations of plant growth regulators. This optimized approach was used to initiate shoot development, promote multiplication, and enhance root formation in the banana plantlets. The protocol also included an effective acclimatization and hardening process using a potting mix of soil. This optimized method resulted in high shoot proliferation rates. The findings contribute to advancing banana cultivar propagation techniques, supporting agricultural efforts to meet the growing demand for bananas, and facilitating the large-scale production of quality banana plants with desirable traits.