Synthesis,characterization and biological evaluation of some new thieno[2,3-d]pyrimidine derivatives

Abstract

Several new thieno[2,3-d]pyrimidine derivatives 2-{[3-chloro-2-(substitutedphenyl)-4-oxoazetidin- 1-yl]amino}-N-(thieno[2,3-d]pyrimidin-4-yl)acetamide 6(a-j), 4-[(2E)-2-(substitutedbenzylidene)hydrazinyl]-2- [(2Z)-2-(2,4-dichlorobenzylidene) hydrazinyl] thieno[2,3-d]pyrimidine 9(a-j) and 3-chloro-4-(substituted phenyl)-1-(thieno[2,3-d]pyrimidin-4-ylamino)azetidin-2-one 14(a-j) were synthesized starting from 2- aminothiophene-3-carbonitrile 1. The characterization of the newly synthesized compounds was established by IR, 1H NMR, 13C NMR and Elemental analysis. The final compounds were screened for their antibacterial activity against Staphylococcus aureus and Streptococcus pyogenes from Gram positive group of bacteria and Escherichia coli and Pseudomonas aeruginosa from Gram negative group of bacteria and antifungal activity against Aspergillus niger.