IFN-γ mRNA expression was evaluated in nonstimulated peripheral blood mononuclear cells (PBMC) of HIV-infected and seronegative individuals using quantitative competitive and semiquantitative RT-PCR and the sensitivity of these methods was compared. A significant correlation was found between quantitative competitive and semiquantitative RT-PCR in samples of both HIV-seronegative (P = 0.004) and HIV-infected individuals (P = 0.0004). PBMC from HIV-infected individuals presented a remarkable increase of IFN-γ mRNA expression, as determined by both types of RT-PCR methods. Semiquantitative RT-PCR even without an internal standard is also acceptable for measuring cytokine mRNA expression, but less reliable if small amounts are quantified. Moreover, we found that increased IFN-γ mRNA expression is independent of CD4+ cell count in AIDS-free HIV-infected patients.
CITATION STYLE
Barabás, É., Nagy, K., Várkonyi, V., & Horváth, A. (2001). Comparison of the quantitative competitive and semiquantitative RT-PCR methods for the determination of interferon-gamma mRNA levels in AIDS-free HIV-infected individuals. Brazilian Journal of Medical and Biological Research, 34(10), 1271–1275. https://doi.org/10.1590/S0100-879X2001001000006
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