Bedrock geology affects foliar nutrient status but has minor influence on leaf carbon isotope discrimination across altitudinal gradients

Abstract

Carbon isotope discrimination (Δ 13 C) in plant leaves generally decreases with increasing altitude in mountains. Lower foliar Δ 13 C at high elevation usually is associated with higher leaf mass per area (LMA) in thicker leaves. However, it is unclear if lower foliar Δ 13 C in high-altitude plants is caused by improved photosynthetic capacity as an effect of higher nutrient, especially nitrogen, content in thicker leaves. We investigated trends of foliar Δ 13 C in four species, each belonging to a different plant functional type (PFT), across two altitudinal gradients, each on a different bedrock type (carbonate and silicate bedrock, respectively) in a region of the southern Alps (Italy) where the foliar Δ 13 C was not affected by water limitation. Our objective was to assess whether the altitudinal patterns of foliar Δ 13 C in relation to leaf morphology and foliar nutrients were conditioned by indirect control of bedrock geology on soil nutrient availability. The foliar Δ 13 C of the four species was mainly affected by LMA and, secondarily, by stomatal density (SD) but the relative importance of these foliar traits varied among species. Area-based nutrient contents had overall minor importance in controlling C discrimination. Relationships among foliar Δ 13 C, foliar nutrient content and leaf growth rate strongly depended on soil nutrient availability varying differently across the two gradients. In the absence of water limitation, the foliar Δ 13 C was primarily controlled by irradiance which can shape anatomical leaf traits, especially LMA and/or SD, whose relative importance in determining C isotope discrimination differed among species and/or PFT. Decreasing foliar Δ 13 C across altitudinal gradients need not be determined by improved photosynthetic capacity deriving from higher nutrient content in thicker leaves.