Production of Exo-polysaccharide by Rhizobium sp.

Abstract

Two fold increase in the yield of glucose and maltose containing exo-polysaccharide (EPS) by Rhizobium sp. was observed during its growth in modified YEMB. EPS production, plant growth promotion activity and root colonization of Rhizobium sp. studies showed enhanced EPS synthesis, more seed germination and over all improvement in plant growth over control and R. meliloti treatment. Groundnut seeds bacterized with Rhizobium sp. resulted in 69. 75% more root length, 49. 51% more shoot height, 13. 75% more number of branches and 13. 60% more number of pods over the control and R. meliloti treatment. Bacterization of wheat seeds increased the dry matter yield of roots (1. 7-fold), and roots adhering soil (RAS) (1. 5) and shoot mass (1. 9-fold). Rhizobium sp. inoculation also increased the population density of EPS-producing bacteria on the rhizoplane. Roots of plants inoculated with Rhizobium sp. maintained a higher K+/Na+ ratio and K+-Na+ selectivity. © 2011 Association of Microbiologists of India.