Holographic microscopy reconstruction in both object and image half-spaces with an undistorted three-dimensional grid

Abstract

We propose a holographic microscopy reconstruction method that propagates the hologram in the object half-space in the vicinity of the object. The calibration yields reconstructions with an undistorted reconstruction grid, i.e., with orthogonal x, y, and z axes and constant pixel pitch. The method is validated with a USAF target imaged by a x60 microscope objective (MO), whose holograms are recorded and reconstructed for different USAF locations along the longitudinal axis: -75 to +75 mum. Since the reconstruction numerical phase mask, the reference phase curvature, and the MO form an afocal device, the reconstruction can be interpreted as occurring equivalently in the object or image half-space.