Effect of IL-10 on the mite antigen-induced IFN-γ production by peripheral blood lymphocytes from individuals with bronchial asthma

Abstract

Interferon-γ (IFN-γ) production was dose-dependently suppressed by the addition of Dermatophagoides farinae (Df), mite antigen in peripheral blood mononuclear cells from children with bronchial asthma. By contrast, the levels of IFN-γ did not change on stimulation with Df antigen in the normal lymphocytes from nonallergic individuals not sensitized with mite. Recombinant interleukin 10 (IL-10), when added to the normal lymphocytes, significantly reduced the production of IFN-γ. Moreover, the decreased synthesis of IFN-γ in patients’ lymphocytes was inversely up-regulated by the treatment with anti-IL-10 antibody. Taken together, results suggest the regulatory role of IL-10 in the secretion of IFN-γ in Df antigen-driven immune responses. © 1995, The Japan Society for Clinical Immunology. All rights reserved.