Effectiveness of Lime Skin Extract (Citrus Amblycarpa) as Natural Larvacide Aedes Aegypti Instar III

Abstract

Various negative effects from the use of chemical larvacides encourage research about natural larvacides, one of which is extract of lime skin extract. This study aimed to determine the effectiveness of lime skin extract as a natural larvacide against the death of Aedes aegypti larvae. This study uses a true experiment design with a post test only control group design. The object of the research was the lime peel extract. The sample size in this study was 450 instar III larvae with Simple Random Sampling technique and treated with various concentrations of lime skin extract (2ml/100ml, 3ml/100 ml, 4ml/100ml, and 5ml/100 ml) for 10 hours. Positive controls use abate 0.01 gr / 100ml. The results showed that all treatment groups could kill 100% of larvae. At a concentration of 4.0 ml / 100ml and 5.0 ml / 100ml experienced 100% mortality after 6 hours of measurement and the positive control group experienced 100% death after 4 hours of measurement. The results of the probit analysis show that the value of LT50 concentration of 5.0% is 2.58 hours and the value of LT99 is 5.86 hours. The Kruskal Wallis test results showed no significant differences (p>0.05) larvae mean mortality between treatment groups and the control group. Giving skin extracts of lime juice can be a natural larvicidal alternative that can be used by the community.