Bioactive compound of Streptomyces capoamus as biocontrol of Bacterial Wilt Disease on Banana Plant

Abstract

Banana is a plant that has a high economic value for human to fulfil his daily fruits need.. The antagonistic test between nine isolates Streptomyces sp. and R. solanacearum showed that the Streptomyces sp. 9 has the highest resistance (19 mm), indicating a strong inhibition and has smallest concentrations of inhibition (MIC) of 10%. The glasshouse scale test demonstrated that the Streptomyces sp. 9 culture sprayed 4 times on a different day could suppress bacterial wilt disease by 100% from 11% in control. The research was to determine molecular identification of Streptomyces sp.9 using 16S rRNA and identification of active compound by Gass Chromatography Mass Spectra (GCMS). The results showed that the molecular identified Streptomyces sp.9 as a Streptomyces capoamus. The chemical compounds which have antimicrobial are Hexadeconoic acid methyl ester, Heneicosane, Hexacosane(CAS) n-Hexacosane, Limonene, Dotriacontane and Tetracontane as antimicrobial agent. The conclusion was Streptomyces capoamus showed various bioactive compounds, which is importance as potential biocontrol on bacterial wilt disease on banana plants. There could be probably of new bioactive compounds and this also provided a new insight towards the development of good candidates for bioactive natural products.