Markers for Sperm Freezability and Relevance of Transcriptome Studies in Semen Cryopreservation: A Review

Abstract

Advances in sperm assessment techniques have offered new perspectives to improve the technology of semen cryopreservation. This review addresses some recent achievements in the proteomics of seminal plasma and spermatozoa and exemplifies its importance as markers for sperm fertility following cryopreservation. Recent advances in transcriptome studies on sperm RNA-Seq data have generated new information aimed to unravel the physiological roles of RNAs in the sperm-egg fertilization processes and their associa-tions with male fertility. The relevance of the sperm freezability markers and the poten-tial associations of RNA-profiling sequences with the sperm biological functions have been discussed.