Chapter 16 Tagging for Protein Expression

Abstract

Tags are frequently used in the expression of recombinant proteins to improve solubility and for affinity purification. A large number of tags have been developed for protein production and researchers face a profusion of choices when designing expression constructs. Here, we survey common affinity and solubility tags, and offer some guidance on their selection and use. © 2009 Elsevier Inc. All rights reserved.