Cytotoxicity Assay Using Brine Shrimp Lethality Test on Collagen-Chitosan Wond Dressing Sterilized by Ultraviolet Light

Abstract

Collagen gives a moist state on the wound area to accelerate the wound healing process. Chitosan is a polymer known as non-toxic, antibacterial, antifungal, biodegradable, and biocompatible material. Combination of collagen and chitosan is expected to be the best biomaterials as a wound dressing for the healing process. The study aimed to determine the cytotoxicity assay on collagen-chitosan wound dressing sterilized by ultraviolet (UV) Light using Brine Shrimp Lethality Test (BSLT) method. The test groups were divided into K0, K1, K2, and K3 groups. K0 contained pure chitosan as a control group, K1 contained collagen 25%-chitosan 75%, K2 contained collagen 50%-chitosan 50%, K3 contained collagen 75%-Chitosan 25%. Collagen was extracted from the skin and scalp of snakehead fish (Channa striata), then it was mixed with chitosan until collagen-chitosan wound dressing formed. This study used Brine Shrimp Level Test (BSLT) method with solution concentration: 10, 50, 100, 250, 500, 750 and 1000 ppm. Based on the results, it showed that K0, K1, K2, and K3 group had LC50 > 1000, proved that collagen-chitosan wound dressing was non-toxic material. The conclusion of the study explain that composite wound dressing based on collagen-chitosan in all groups that was sterilized under UV-Light for 15 minutes was not toxic. Also, it showed LC50 > 1000 based on Brine Shrimp Lethality Test.