The Role of Saccharomyces boulardii as a Probiotic in Mice with Celiac Disease

Abstract

Saccharomyces boulardii is used as a probiotic with the purpose of introducing beneficial microbes into the intestines and protection against pathogens. The goal of this study is to see if it can prevent mice from celiac disease. 20 mice were divided into four groups as follows: control, disease (1.5 mg/g gliadin oral), co-treatment (S. boulardii 106 cfu/kg oral after gliadin gavage) and S. boulardii 106 cfu/kg groups. Tissue transglutaminase IgA (tTg-IgA), pepsin, amylase, vitamin D3 was measure by enzyme-linked immunosorbent assay (ELISA). Analytical statistics The data were compared using ANOVA. Results shown the tTG-IgA was increase in the group disease but was decrease in co-treatment (S. boulardii 106 cfu/kg oral after gliadin gavage) and S. boulardii 106 cfu/kg groups. Pepsin and vitamin D3 had decreased in group disease, However, in the co-treatment group, things returned to normal. Amylase levels in the disease group were greater than in the control group, Between the co-treatment and control groups, there was a decrease. We found that giving S. boulardii to those with celiac disease improved their biochemical, which was more noticeable after they were exposed to gliadin.